A simple method for isolating disomic strains ofSaccharomyces cerevisiae

A simple method for isolating individual microbes.

Mitotic chromosome loss in a disomic haploid of Saccharomyces cerevisiae.

Experiments designed to characterize the incidence of mitotic chromosome loss in a yeast disomic haploid were performed; The selective methods employed utulize the non-mating property of strains disomic for linkage group III and heterozygous at the mating type locus. The principal findings are: (1) The grequency of spontaneous chromosome loss in the disome is of the order 10- minus 4 per cell; ...

A New Method for Isolating I-(+)-lysine

None of the methods which have been used for isolating Z-(+)lysine from plant extracts and protein hydrolysates is ideal. The oldest method utilizes phosphotungstic acid to precipitate the basic nitrogenous compounds which must be fractionated subsequently (1, 2). In the Kossel-Kutscher silver sulfate-barium hydroxide fractionation (3) the lysine remaining in the final filtrate must be precipit...

A rapid method for isolating glandular trichomes.

A physical method is described for the rapid isolation of plant trichomes, with emphasis on stalked glandular types. The technique involved breaking frozen trichomes with powdered dry ice and collection of glandular heads by sieving from larger tissue fragments. This method was applied to several plants that bear similar stalked trichomes: geranium (Pelargonium), potato (Solanum tuberosum), tom...

A New Method for Isolating I-(+)-lysine

None of the methods which have been used for isolating Z-(+)lysine from plant extracts and protein hydrolysates is ideal. The oldest method utilizes phosphotungstic acid to precipitate the basic nitrogenous compounds which must be fractionated subsequently (1, 2). In the Kossel-Kutscher silver sulfate-barium hydroxide fractionation (3) the lysine remaining in the final filtrate must be precipit...